The methanol extracts inhibited most of strains at tested concentrations while the ether extract was active only against s. In vitro antimalarial activity and cytotoxicity of some. It appears to be the mostused plant in the genus vernonia. Vernonia amygdalina va is a woody shrub that can grow up to 5 m tall, belonging to the family of asteraceae 3.
Phytochemical analysis, analgesic and antipyretic properties. In vivo and in vitro antimalarial activity of vernonia amygdalina. Antileukemia activity from root cultures of vernonia amygdalina mutasim m. Sisodia 1 department of biochemistry, faculty of life sciences, university of benin, pmb 1154, benin city, nigeria 2 in vivo animal testing facility, central institute of medicinal and aromatic plants, lucknow, india.
In vivo antimalarial activity of vernonia amygdalina. Antiplasmodial activity of ethanol extract of vernonia amygdalina leaf in plasmodium doi. The aim of this study was to assess the activity of vernonia amygdalina leaf extracts and isolated. Sep 18, 2008 antimalarial activity of hydromethanolic extract and its solvent fractions of vernonia amygdalina leaves in mice infected with plasmodium berghei may 2019 sage open medicine, vol. Antitumoral and antimicrobial activities of bitter sesquiterpene lactones of vernonia amygdalina, a possible medicinal plant used by wild chimpanzees. Evaluation of the antimalarial effect of the methanolic leaf. Transactions of the royal society of tropical medicine and hygiene 01 nov 2000, 946. Antimalarial activity of hydromethanolic extract and its solvent fractions of vernonia amygdalina leaves in mice infected with plasmodium berghei article pdf available may 2019 with 60 reads. In vitro antimalarial and cytotoxic activities of leaf extracts of vernonia amygdalina del.
Antileukemia activity from root cultures of vernonia. Antimalarial activity of hydromethanolic extract and its solvent fractions of vernonia amygdalina leaves in mice infected with plasmodium berghei may 2019 sage open medicine, vol. Moreover, vernonia amygdalina can be used for the treatment of malaria in rural communities. The young leaves are used as an antihelmintic, antimalarial, laxativepurgative, enema, worm expeller and fertility inducer in subfertile women. The presence and effect of these compounds validate the use of this plant for the treatment of malaria in the traditional medicinal practice of nigeria. The leaves exhibit a characteristic odor and bitter taste, explaining.
Leaf, medium to dark green, with or without sparse hairs above, with fine, soft, pale hairs below and conspicuous redveining. A search of cuban medicinal species with antimalarial activity is being developed in our laboratory. Issn 15976343 in vitro antimalarial activity of the extracts of vernonia amygdalina prepare thick and thin smears on clean slides for each patient. Evaluation of the antimalarial effect of the methanolic. Antimalarial activity of medicinal plants used in traditional medicine in s. The analgesic and antiplasmodial activities and toxicology. The study was aimed at assessing the effect of cooking egusisoup on the antibacterial activity of bitter leaf vernonia amygdalina. The antimalarial activity of the test extract was expressed as 50% inhibitory concentration ic 50 determined from doseresponse curve by nonlinear regression analysis curvefit using graph pad prism. Sunday nweke of the department of pharmacognosy, university of benin, nigeria. Vernonia amygdalina protected against carbon tetrachlorideinduced liver injury by inducing antioxidant and phase 2 enzymes. This study describes the in vivo antimalarial activity of v. Medicinal plants are a validated source for discovery of new leads and standardized herbal medicines. A standard inoculum of 1 x 10 7 infected erythrocytes is used, and leaf and rootbark extracts of 500 mgkg, 250 mgkg or 125 mgkg are used in a fourday suppression test and a rane test of established infection.
These compounds showed antimalarial activity at different levels. Bioactive compounds in vernonia amygdalina several studies had been done in isolating and characterizing some bioactive compounds from v. In vivo and in vitro antimalarial activity of vernonia amygdalina leaves extracts. Vernonia amygdalina is traditionally used in ethiopia to treat various diseases.
Antimalarial activity of hydromethanolic extract and its solvent fractions of vernonia amygdalina leaves in mice infected with plasmodium berghei article pdf. Plasmodium transmission blocking activities of vernonia. The anticancer activities of vernonia amygdalina extract. Phytochemical analysis of vernonia amygdalina leaves showed that anthracene, glycosides, steroids, flavonoids, proteins, carbohydrates, reducing sugars, saponins and tannins were present 26.
The chcl 3 extract after silica gel column chromatography has led to the isolation of two compounds identified as tricosane 1 and vernolide 2, while the acetone extract furnished isorhamnetin 3 and luteolin. The analgesic and antiplasmodial activities and toxicology of. Antimalarial activity of hydromethanolic extract and its. Vernonia amygdalina possesses several bioactive compounds and is used in traditional medicines of southwestern uganda, along with other regions.
In vivo antimalarial activity of vernonia amygdalina researchgate. The leaves are consumed either as a vegetable or as aqueous extracts used as tonics for the treatment of various illnesses 9. This study evaluated the in vivo antiplasmodial, antioxidant and immunomodulatory effect of ethanol extract of v. Effect of methanolic extract of vernonia amygdalina common. Ninetynine plant species in brazil were reportedly claimed to have antimalarial activity milliken, 1997.
Phytochemical and pharmacological prope of vernonia. Antimicrobial activities of vernonia amygdalina del and. Antileukemia activity from root cultures of vernonia amygdalina. Its analgesic potential has not been investigated thus far. The antimalaria activity of vernonia amygdalina del. Diabetes control and treatment vernonia amygdalina contains biologicallyactive compounds known as coumarins, flavonoids, anthraquinone, phenolic acids, saponins, sesquiterpenes, xanthones, terpenes, steroids, lignans and edotides. Antimalarial activity was detected in both extracts. Evaluation of in vivo antiplasmodial activities of. The antimicrobial activity test of the apex shoot of vernonia amygdalina extract showed activities against some strains.
Nov 29, 2016 the results of this study show that aqueous leaf extracts of v. This prompted us to isolate bioactive compounds from the flowers of this plant. Preliminary phytochemical screening of the nhexane, ethanol. In the present study, the antimicrobial activity of aqueous and ethanol extracts of rwandan plants vernonia aemulans, vernonia amygdalina, lantana camara and markhamia lutea leaves were. The antimalarial activity of vernonia amygdalina del. Vernonia amygdalina va, commonly called bitterleaf, is widely cultivated in africa and is reported to have a wide spectrum of medicinal applications including antidiabetic, antimalarial and antihelmintic abosi and raseroke, 2003. The number of mice used for the toxicity test was 20 5group and for each extract and control groups 5 mice per group. This study thus sought to evaluate the in vivo antimalarial activity of ethanolic and aqueous leaf extracts of vernonia amygdalina on plasmodium berghei infected mice. It is a perennial plant with height between 1 m and 6 m nwosu et al. Comparative chemical analyses of vernonia amygdalina and.
The present study examines the antinociceptive potential of the aqueous leaf extract 50200 mgkg using three models of nociception acetic acid. Table 4 phytochemical analysis of the various plant parts of morinda morindiodes plant parts quantity of compound investigated compounds alkaloid g100g saponin g100g tannin mg100g flavonoid mg100g glycocyanides mg100g abosi ao, raseroka bh. The aim of this study was to assess the activity of vernonia amygdalina leaf extracts and isolated compounds against gametocytes and sporogonic stages of plasmodium berghei and to validate the findings on field isolates of plasmodium falciparum. Preliminary phytochemical screening of the nhexane. Evaluation of antipyretic and analgesic effects of aqueous. Ethnobotanical information indicated that more than 800 plants including vernonia amygdalina are used as traditional remedies for the treatment of diabetes due to their effectiveness, less side effects and low cost rathjod et al. In vitro antimalarial and cytotoxic activities of leaf. Antimalarial, halfmaximal inhibitory concentration, isolation, phytocompounds, vernonia amygdalina.
The antimalarial activities of acetonewater and aqueous extracts of vernonia amygdalina green leaves were tested on a chloroquinesensitive isolate of plasmodium falciparum. Asteraceae is reported to be traditionally used for the treatment of malaria. Vernonia amygdalina is a valuable medicinal plant that is widespread in west africa, it is known as bitter leaf due to its characteristic bitter taste and flavour, and canbe used as an active anticancer, antibacterial, antimalarial and antiparastic agent. It can also act as a repellant against certain bugs, nematodes, aphids and insects. Antimicrobial activity of vernonia amygdalina justicia. Antibacterial activity of bitter leaf amygdalina soup on. Antidiabetic effect of combined aqueous leaf extract of vernonia amygdalina and metformin in rats, u adikwu michael, b uzuegbu david, c okoye theophine, f uzor philip, adibe maxwel. Phytochemical screening and antibacterial activity of. Native to nigeria west africa and widely grown in africa 3, va is also found in asia, and is especially common in singapore and malaysia 4,5. However, current research about the mechanisms of the anti. Antiplasmodial, antioxidant and immunomodulatory activities. The leaves are green with a characteristic odour and a bitter taste.
The study determined if administration of vernonia amygdalina and carica papaya plants provides synergistic effects in ameliorating plasmodium infection in mice. An overview of evidence and pharmacology 169 produces large mass of forage and is drought to lerant hutchioson and dalziel, 1963, it is 2 5 m with petiolate leaf of about 6 mm diamet er and elliptic shape. African traditional beers are both considered as food and beverages for african people and hence preserving them using the natural additive is of utmost importance. The number of mice used for the toxicity test was 20 5group and for each extract and control groups 5 mice per group was used. Based on folkloric repute of this plant in ethiopian traditional medicine and crude extractbased ethnopharmacological studies conducted in few countries, this study was undertaken to evaluate the in vivo antimalarial activity of 80% methanol extract and its solvent fractions of the leaves. In vivo and in vitro antimalarial activity of vernonia. Phytochemical analysis of vernonia amygdalina and ocimum. The chcl 3 extract after silica gel column chromatography has led to the isolation of two compounds identified as tricosane 1 and vernolide 2, while the acetone extract furnished isorhamnetin 3 and luteolin 4. Vernonia amygdalina, popularly known as bitter leaf, is an under shrub of variable height with petiolate green leaves of about 6mm diameter. Antiplasmodial activity of vernonia adoensis aqueous. A clinical trial of the traditional medicine vernonia. Notably, medicinal plant extracts may be a potential source for treatments of breast cancer. Antimalarial potential of carica papaya and vernonia. Phytochemical screening and antibacterial activity of bitter.
Using spectroscopic techniques, the study had isolated and. The present study determines the ability of vernonia amygdalina leaf extract to enhance the prophylactic and. Some physical properties of vernonia amygdalina and garcinia. Bitter leaves wood ash can be used to treat and control seedborne fungi. Antimalarial activity of hydromethanolic extract and its solvent. The phytochemical studies had resulted in the isolation of flavonoids, saponins, alkaloids, tannins, phenolics, terpenes, steroidal glycosides. The observation that an apparently sick wild chimpanzee chewed v. Effects of single and combined exposure of albino rats to. Vernonia amygdalina with the aim of verifying the antibacterial activities of the plant so as to find an alternative for the common antibiotics present in use and aslo to detect the dosage and toxicological effect of the plant. Extracts from the leaves and root bark of vernonia amygdalina are assessed for antimalarial activity against drugsensitive plasmodium berghei in mice. The study was aimed to determine the phytochemical constituents and antibacterial activity of vernonia amygdalina leaf extracts against some bacteria associated with gastroenteritis. The leaves of the shrub vernonia amygdalina del compositae are widely used in africa to treat malaria. Antibacterial and antioxidant compounds from the flower. The phytochemical analysis of azadirachta indica revealed higher concentrations mg100g of alkaloids, glycosides, phenols, steroids and minimal levels of other phytochemicals fig.
Effect of methanolic extract of vernonia amygdalina. Vernonia amygdalina va is a woody shrub reported to have not only diverse therapeutic effects but also anticancer properties. Antidiabetic effect of combined aqueous leaf extract of. Vernonia amygdalina and its saponins have been reported to elicit antitumoral activities in leukemia cells 25. Review on malaria and antimalarial activity of vernonia. This finding confirms the work of jegede 2009, unpublished which similarly observed the antimalarial activity of vernonia amygdalina at same dosage and. Current perspectives on the medicinal potentials of. Antiplasmodial, antioxidant and immunomodulatory activities of. The leaf of carica papaya in combination with vernonia amygdalina is used traditionally to treat infection 7, 9. Antitumoral and antimicrobial activities of bitter. Group 1 was normal control, while groups 26 were intraperitoneally inoculated 2. In traditional medicine, herbal practitioners use v. Pdf antimalarial activity of hydromethanolic extract and. Extracts of the leaves of bitterleaf plant were made using ethanol, from bitterleaf soup and using hot water.
The amazing health benefits of bitter leaf vernonia. Antioxidative and chemopreventive properties of vernonia. The antibacterial activity of the extracts was tested using two pathogenic hospital isolates. Pdf antimalarial activity of hydromethanolic extract and its solvent. In vitro antimalarial activity of the extracts african journals online. Figure 1 reveals the percentage of parasitized erythrocytes of mice infected with p. The plants have been proven in human medicine to possess antimalaria and antihelminthic properties abosi and raseroka, 2003. In nigeria, over 100 plant species including vernonia cinera and vernonia amygdalina are used in herbal medicine as remedies for malaria adebayo and krettli, 2010. Invivo antimalarial activity of aqueous and ethanolic extracts of vernonia amygdalina on plasmodium berghei infected mice. Oct 17, 2018 the aim of this study was to investigate the antiplasmodial effects of the crude aqueous, methanol and chloroform extracts of the leaves of vernonia adoensis in plasmodium berghei infected swiss albino mice using peters 4day suppressive test. Hence, the activities of the extracts, especially the methanol fraction could. Vernonia amygdalina del asteraceae is a small shrub with dark green leaves and rough barks growing predominantly in tropical africa but has been domesticated in many parts of west africa igile et al.
Antiplasmodial activity of ethanol extract of vernonia. Novel phytocompounds from vernonia amygdalina with. The aim of this study was to investigate the antiplasmodial effects of the crude aqueous, methanol and chloroform extracts of the leaves of vernonia adoensis in plasmodium berghei infected swiss albino mice using peters 4day suppressive test. Vernonia amygdalinaare green with a char acteristic odour and bitter taste. Enhanced antimalarial effects of chloroquine by aqueous. Vernonia amygdalina leaves contain molecules affecting multiple. Products with antimalarial activity have been isolated from plants used against other parasites5,10 and some antimalarial natural products have shown good activity against a broad spectrum of parasites1.
This study examined the efficacy and safety of an infusion of fresh v. Elshemy2 1commission for biotechnology and genetic engineering, p. Vernonia amygdalina leaves and seeds of garcinia kola were bought from a local market in benin city, edo state, nigeria and identified by mr. The antioxidant activity of vernonia amygdalina has been attributed to the presence of flavonoids, as reported by igile et al. Antimalarial activity of medicinal plants used in traditional. Health benefits of bitter leaf vernonia amygdalina the leaves are widely used as a remedy for fever and are known as quinine substitute in nigeria.
Vernonia amygdalina popularly known as bitter leaf is a shrub of 25m tall with petiolate green leaves of about 6 mm diameters which are characteristically bitter ojiako and nwayo, 2006. It is widely available, accessible, and affordable in many remote areas that do not have ready access to modern medicines. A 4day suppressive test was conducted on mice infected with p. Medicinal it is used as a medicine for relieving fevers, stomach disorders, to treat hepatitis, malaria, bilharzias, spots on skin and nausea. Antimalarial activity of hydromethanolic extract and its solvent fractions of vernonia amygdalina leaves in mice infected with plasmodium berghei article pdf available in sage open 7. Based on folkloric repute of this plant in ethiopian traditional medicine and crude extractbased ethnopharmacological studies conducted in few countries, this study was undertaken to evaluate the in vivo antimalarial activity of 80% methanol extract and its solvent. Antimicrobial activity and phytochemicals analysis of. Vernonia amygdalina family, compositae is a vegetable shrub that is widely spread in east. Scientists reveal that bitter leaves extract helps to reduce the blood estrogen level in the body by suppressing the activities of aromatase. Breast cancer is currently the leading cause of cancerrelated deaths among women globally. Food bitter leaf eaten as raw vegetables and cooked in soups.
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